Rabiller, Matthias:
Interactions of SUMO proteins
Duisburg, Essen, 2005
2005dissertation
BiologyFaculty of Biology
Title in English:
Interactions of SUMO proteins
Author:
Rabiller, Matthias
Thesis advisor:
Bayer, PeterUDE
GND
1059319691
LSF ID
10134
ORCID
0000-0003-0435-7202ORCID iD
Other
connected with university
Place of publication:
Duisburg, Essen
Year of publication:
2005
Extent:
82 S. : graph. Darst.
DuEPublico 1 ID
Library shelfmark:
Note:
Duisburg, Essen, Univ., Diss., 2006
Language of text:
English

Abstract in English:

A large number of proteins have shown ability to bind to SUMO (Small Ubiquitin like Modifier) proteins through a short conserved motif called SIM (SUMO Interacting Motif). The work presented here shows that the different SUMO isoforms interact with the hydrophobic core of the SIM by forming an intermolecular b-sheet with the b2 strand of SUMO. This interaction is crucial for SUMO binding, and is modulated by interactions between SUMO and the amino acids flanking the core of the SIM. The SIM can be phosphorylated, providing a possibility for regulating the strength of SUMO binding in the lifetime of a protein. Furthermore, a concentration threshold effect is observed in the binding of the unphosphorylated SIM of PIAS (Protein inhibitor of activated STAT) to SUMO. The dependency on the amino acids flanking the hydrophobic core is stronger in binding to SUMO1 than to SUMO2, providing a mechanism for SUMO isoform discrimination.