Hauske, Patrick; Mamant, Nicolette; Hasenbein, Sonja; Nickel, Sabrina; Ottmann, Christian; Clausen, Tim; Ehrmann, Michael; Kaiser, Markus:
Peptidic small molecule activators of the stress sensor DegS
In: Molecular BioSystems, Jg. 5 (2009), Heft 9, S. 980 - 985
2009Artikel/Aufsatz in Zeitschrift
BiologieFakultät für BiologieForschungszentren » Zentrum für Medizinische Biotechnologie (ZMB)
Damit verbunden: 1 Publikation(en)
Titel in Englisch:
Peptidic small molecule activators of the stress sensor DegS
Autor*in:
Hauske, PatrickUDE
LSF ID
53368
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Mamant, Nicolette;Hasenbein, Sonja;Nickel, SabrinaUDE
LSF ID
53369
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Ottmann, Christian;Clausen, Tim;Ehrmann, MichaelUDE
LSF ID
13331
ORCID
0000-0002-1927-260XORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Kaiser, MarkusUDE
LSF ID
52590
ORCID
0000-0002-6540-8520ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2009
Sprache des Textes:
Englisch

Abstract:

Bacterial DegS is a regulatory protease that acts as a molecular stress sensor and initiates a periplasmic stress response pathway. Upon binding of misfolded proteins to its PDZ domain, the protease domain of DegS is allosterically activated, thereby initiating a signal cascade that results in the elevated expression of protein quality control factors. Although the structural basis of this activation mode has been elucidated previously, it is not yet fully understood if binding to the PDZ domain is sufficient for protease domain activation or if secondary interactions with the protease domain are required. Here, we demonstrate that tripeptidic small molecule activators which only bind to the PDZ domain are sufficient to trigger DegS activation . Furthermore, we show that the hydrophobicity of the peptidic small molecule activators is a critical determinant for efficient activation .