Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
In: Research in Microbiology, Vol. 154 (2003), No. 9, pp. 654 - 657
2003article/chapter in journal
BiologyFaculty of BiologyScientific institutes » Center of Medical Biotechnology (ZMB)
Related: 1 publication(s)
Title:
Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
Author:
Ehrle, Rainer;Mikhaleva, Natasha;Boyd, Dana;Davidson, Amy L.;Ehrmann, MichaelUDE
- LSF ID
- 13331
- ORCID
- 0000-0002-1927-260X
- Other
- connected with university
Year of publication:
2003
Abstract:
Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.