Rose, Rolf; Huttenlocher, Franziska; Cedzich, Anna; Kaiser, Markus; Schaller, Andreas; Ottmann, Christian:
Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
In: Acta crystallographica, Section F, Structural biology and crystallization communications online, Jg. 65 (2009), Heft 5, S. 522 - 525
2009Artikel/Aufsatz in Zeitschrift
BiologieFakultät für Biologie
Titel:
Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
Autor*in:
Rose, Rolf;Huttenlocher, Franziska;Cedzich, Anna;Kaiser, MarkusUDE
LSF ID
52590
ORCID
0000-0002-6540-8520ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Schaller, Andreas;Ottmann, Christian
Erscheinungsjahr:
2009

Abstract:

The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.