Cao, Kan; Nakajima, Reiko; Meyer, Hemmo; Zheng, Yixian:
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis
In: Cell, Vol. 115 (2003), No. 3, pp. 355 - 367
2003article/chapter in journal
Biology
Related: 1 publication(s)
Title:
The AAA-ATPase Cdc48/p97 regulates spindle disassembly at the end of mitosis
Author:
Cao, Kan;Nakajima, Reiko;Meyer, HemmoUDE
GND
1089446616
LSF ID
51479
ORCID
0000-0003-1883-1796ORCID iD
Other
connected with university
;
Zheng, Yixian
Year of publication:
2003

Abstract:

Spindle disassembly at the end of mitosis is a complex and poorly understood process. Here, we report that the AAA-ATPase Cdc48/p97 and its adapters Ufd1-Npl4, which have a well-established role in membrane functions, also regulate spindle disassembly by modulating microtubule dynamics and bundling at the end of mitosis. In the absence of p97-Ufd1-Npl4 function, microtubules in Xenopus egg extracts remain as monopolar spindles attached to condensed chromosomes after Cdc2 kinase activity has returned to the interphase level. Consequently, interphase microtubule arrays and nuclei are not established. Genetic analyses of Cdc48, the yeast homolog of p97, reveal that Cdc48 is also required for disassembly of mitotic spindles after execution of the mitotic exit pathway. Furthermore, Cdc48/p97-Ufd1-Npl4 directly binds to spindle assembly factors and regulates their interaction with microtubules at the end of mitosis. Therefore, Cdc48/p97-Ufd1-Npl4 is an essential chaperone that regulates transformation of the microtubule structure as cells reenter interphase.