Tielen, Petra; Strathmann, Martin; Jaeger, Karl-Erich; Flemming, Hans-Curt; Wingender, Jost:
Alginate acetylation influences initial surface colonization by mucoid Pseudomonas aeruginosa
In: Microbiological Research, Jg. 160 (2005), Heft 2, S. 165 - 176
2005Artikel/Aufsatz in ZeitschriftOpen Access
Chemie
Titel in Englisch:
Alginate acetylation influences initial surface colonization by mucoid Pseudomonas aeruginosa
Autor*in:
Tielen, PetraUDE
LSF ID
11409
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Strathmann, MartinUDE
LSF ID
14744
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Jaeger, Karl-Erich;Flemming, Hans-CurtUDE
LSF ID
11401
ORCID
0000-0002-3670-9236ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Wingender, JostUDE
LSF ID
11404
ORCID
0000-0003-3143-4760ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2005
Open Access?:
Open Access
Sprache des Textes:
Englisch

Abstract:

Mucoid strains of Pseudomonas aeruginosa overproduce the exopolysaccharide alginate, which is substituted with O-acetyl groups. Under nongrowing conditions in phosphate buffer, a mucoid clin. strain formed microcolonies on steel surfaces, while an acetylation-defective mutant was unable to form cell clusters. Enzymic degrdn. of alginate by alginate lyase prevented microcolony formation of the mucoid parent strain. In a continuous culture flow-cell system, using gluconate minimal medium, the mucoid strain with acetylated alginate formed microcolonies and grew into heterogeneous biofilms, whereas the acetylation-defective mutant produced a thinner and more homogeneous biofilm. A lowered viscosity of extracellular material from the acetylation-defective mutant indicated a weakening of exopolymer interactions by loss of acetyl groups. These results suggest that acetyl substituents are necessary for the function of high-mol.-mass alginate to mediate cell aggregation into microcolonies in the early stages of biofilm development by mucoid P. aeruginosa, thereby detg. the architecture of the mature biofilm.