Ehrle, Rainer; Mikhaleva, Natasha; Boyd, Dana; Davidson, Amy L.; Ehrmann, Michael:
Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
In: Research in Microbiology, Jg. 154 (2003), Heft 9, S. 654 - 657
2003Artikel/Aufsatz in Zeitschrift
BiologieFakultät für BiologieForschungszentren » Zentrum für Medizinische Biotechnologie (ZMB)
Damit verbunden: 1 Publikation(en)
Titel:
Context-dependent effects of charged residues in transmembrane segments of MalF-PhoA fusions
Autor*in:
Ehrle, Rainer;Mikhaleva, Natasha;Boyd, Dana;Davidson, Amy L.;Ehrmann, MichaelUDE
LSF ID
13331
ORCID
0000-0002-1927-260XORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2003

Abstract:

Charged residues were introduced into the C-terminal transmembrane segment of a MalF–PhoA fusion to investigate the efficiency of the altered transmembrane segment to function as an export signal or a stop transfer signal. PhoA assays revealed that charges had negative effects when the transmembrane segment was part of a stop transfer signal but not when it was part of an export signal. Implications of this finding for the biogenesis of polytopic membrane proteins are discussed.