Steinke, Angelika; Grau, Sandra; Davidson, Amy; Hofmann, Eckhard; Ehrmann, Michael:
Characterization of transmembrane segments 3, 4, and 5 of Ma1F by mutational analysis
In: Journal of Bacteriology, Jg. 183 (2001), Heft 1, S. 375 - 381
2001Artikel/Aufsatz in Zeitschrift
BiologieFakultät für Biologie
Damit verbunden: 1 Publikation(en)
Titel:
Characterization of transmembrane segments 3, 4, and 5 of Ma1F by mutational analysis
Autor*in:
Steinke, Angelika;Grau, Sandra;Davidson, Amy;Hofmann, Eckhard;Ehrmann, MichaelUDE
LSF ID
13331
ORCID
0000-0002-1927-260XORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2001

Abstract:

Ma1F and Ma1G are the cytoplasmic membrane components of the binding protein-dependent ATP binding cassette maltose transporter in Escherichia coli. They are thought to form the transport channel and are thus of critical importance for the mechanism of transport. To study the contributions of individual transmembrane segments of Ma1F, we isolated 27 point mutations in membrane-spanning segments 3, 4, and 5. These data complement a previous study, which described the mutagenesis of membrane-spanning segments 6, 7, and 8. While most of the isolated mutations appear to cause assembly defects, L323Q in helix 5 could interfere more directly with substrate specificity. The phenotypes and locations of the mutations are consistent with a previously postulated structural model of Ma1F.