Patak, Pauline; Jin, Fengyan; Schäfer, Simon; Metzen, Eric; Hermann, Dirk:
The ATP-binding cassette transporters ABCB1 and ABCC1 are not regulated by hypoxia in immortalised human brain microvascular endothelial cells
In: Experimental and Translational Stroke Medicine, Jg. 3 (2011), Heft 12
2011Artikel/Aufsatz in ZeitschriftOA Gold
MedizinMedizinische Fakultät » Universitätsklinikum Essen » Klinik für NeurologieForschungszentren » Zentrum für Medizinische Biotechnologie (ZMB)Medizinische Fakultät » Universitätsklinikum Essen » Institut für Physiologie
Damit verbunden: 1 Publikation(en)
Titel in Englisch:
The ATP-binding cassette transporters ABCB1 and ABCC1 are not regulated by hypoxia in immortalised human brain microvascular endothelial cells
Autor*in:
Patak, Pauline;Jin, Fengyan;Schäfer, SimonUDE
LSF ID
12996
ORCID
0000-0001-7289-6000ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Metzen, EricUDE
LSF ID
50408
ORCID
0000-0002-2740-3219ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
;
Hermann, DirkUDE
GND
124495648
LSF ID
50474
ORCID
0000-0003-0198-3152ORCID iD
Sonstiges
der Hochschule zugeordnete*r Autor*in
Erscheinungsjahr:
2011
Open Access?:
OA Gold
DuEPublico 1 ID
EVALuna Biblio ID
19582
PubMed ID
Notiz:
OA Förderung 2012
Sprache des Textes:
Englisch

Abstract in Englisch:

Background: ATP-binding cassette transporters at the blood-brain barrier are actively regulated upon ischemic stroke in a way that impedes the access of pharmacological compounds to the brain tissue. The luminal endothelial transporter ABCB1 was recently shown to be increased, whereas the abluminal transporter ABCC1 was decreased on ischemic brain capillaries. In vitro studies using epithelial cells suggested that ABCB1 is regulated during hypoxia in a hypoxia-inducible factor (HIF)-1a-dependent way. Methods: In order to investigate whether hypoxia might be responsible for the expression changes of ABCB1 and ABCC1 in the ischemic brain, the immortalised human brain microvascular endothelial cell line hCMEC/D3 was exposed to hypoxia (1%) or anoxia (0%). Cell lysates were analysed by Western blot to detect the protein expression of ABCB1, ABCC1, HIF-1a and HIF-2a. Results: During hypoxia, an accumulation of HIF-1a and HIF-2a was noticed in hCMEC/D3 cells that followed different time kinetics. Both HIF-1a and HIF-2a abundance increased within 4 h of hypoxia. HIF-1a levels decreased to below detection levels within 16 h of hypoxia, whereas HIF-2a remained elevated even after 48 h. No changes of ABCB1 and ABCC1 expression were detected, neither on the mRNA nor protein level. Conclusion: Our data suggests that other factors than