Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
In: Acta crystallographica, Section F, Structural biology and crystallization communications online, Vol. 65 (2009), No. 5, pp. 522 - 525
2009article/chapter in journal
BiologyFaculty of Biology
Title:
Purification, crystallization and preliminary X-ray diffraction analysis of a plant subtilase.
Author:
Rose, Rolf;Huttenlocher, Franziska;Cedzich, Anna;Kaiser, MarkusUDE
- LSF ID
- 52590
- ORCID
- 0000-0002-6540-8520
- Other
- connected with university
Year of publication:
2009
Abstract:
The subtilase SBT3 from Solanum lycopersicum (tomato) was purified from a tomato cell culture and crystallized using the sitting-drop vapour-diffusion method. A native data set was collected to 2.5 A resolution at 100 K using synchrotron radiation. For experimental phasing, CsCl-derivative and tetrakis(acetoxymercuri)methane (TAMM) derivative crystals were employed for MIRAS phasing. Three caesium sites and one TAMM site were identified, which allowed solution of the structure.