- GND
- 130722069X
- LSF ID
- 59461
- ORCID
- 0000-0002-9413-846X
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
korrespondierende*r Autor*in
- LSF ID
- 51606
- ORCID
- 0000-0003-4321-0924
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
- GND
- 124651291
- LSF ID
- 49829
- ORCID
- 0000-0001-6062-0357
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
korrespondierende*r Autor*in
- LSF ID
- 54494
- ORCID
- 0000-0002-9409-1550
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
Abstract in Englisch:
A transfection vector based on a peptide dendrimer (1) has been developed and its abilities for DNA binding and transport have been investigated. By attaching a fluorophore to the vector system (1*), several steps in the transfection process could be monitored directly. As DLS and AFM studies showed, the labeled vector 1* condensed DNA into tightly packed aggregates able to enter eukaryotic cells. Co-localization experiments revealed that the ligand/plasmid complex is taken up by the endosomal pathway followed by an endosomal escape or lysosomal degradation. Afterwards, the plasmid DNA seems to enter the nucleus due to a breakdown of the nuclear envelope during mitosis, as only cells that have recently undergone mitosis showed H2B-GFP expression.