- ORCID
- 0000-0003-4194-8108
- Sonstiges
- korrespondierende*r Autor*in
- ORCID
- 0000-0001-5293-4292
- ORCID
- 0000-0002-1451-6273
- LSF ID
- 57733
- ORCID
- 0000-0003-2362-8784
- Sonstiges
- der Hochschule zugeordnete*r Autor*in
korrespondierende*r Autor*in
Abstract in Englisch:
Chromosome biorientation on the mitotic spindle is prerequisite to errorless genome inheritance. CENP-E (kinesin-7) and dynein–dynactin (DD), microtubule motors with opposite polarity, promote biorientation from the kinetochore corona, a polymeric structure whose assembly requires MPS1 kinase. The corona's building block consists of ROD, Zwilch, ZW10, and the DD adaptor Spindly (RZZS). How CENP-E and DD are scaffolded and mutually coordinated in the corona remains unclear. Here, we show that when corona assembly is prevented through MPS1 inhibition, CENP-E is absolutely required to retain RZZS at kinetochores. An RZZS phosphomimetic mutant bypasses this requirement, demonstrating the existence of a second receptor for polymeric RZZS. With active MPS1, CENP-E is dispensable for corona expansion, but strictly required for physiological kinetochore accumulation of DD. Thus, we identify the corona as an integrated scaffold where CENP-E kinesin controls DD kinetochore loading for coordinated bidirectional transport of chromosome cargo.